CytoFlora® for Children: Gut Health Insights on Behavior and Communica – Bioray

By Stephanie Ray, BCN

Naturopath, Formulator, and Mom

Using CytoFlora®, this case highlights supportive changes in a child’s neurological function, natural detoxification processes, and healthy mitochondrial activity.*

Organic Acid Testing (OAT) was used to evaluate before-and-after intake results in a 5-year-old boy. OAT helps identify patterns related to gut balance, nutrient absorption, and detoxification capacity.* CytoFlora®, a liquid probiotic lysate formula, was used to support healthy gut terrain and microbial balance while supporting nutrient absorption and gentle detoxification support.*

Overview of the Case

Before beginning CytoFlora®, the child’s caregivers reported defiance, temper tantrums, difficulty making and using grammatically correct sentences, and a diminished ability to communicate overall.

microbial metabolites from an imbalanced gut terrain,*
oxalate stress,*
mitochondrial activity markers,*
neurotransmitter-related metabolites,*
and reduced indicators of detoxification support.*

After 3 months of daily CytoFlora®, the child’s OAT showed improvement in 75 of 77 areas measured. Caregivers observed easier communication, fewer outbursts, improved cooperation, and calmer behavior. Laboratory patterns also reflected better detoxification support and more efficient nutrient utilization.*

From a traditional Chinese herbal viewpoint, supporting the gut, reducing dampness, and calming internal agitation often aligns with improvements in digestion, behavior, and language development.

What Is the Organic Acid Test (OAT)?

The Organic Acid Test is a simple urine test that helps evaluate how well the body is:

using nutrients,*
supporting energy production,*
processing microbial byproducts,*
and clearing everyday metabolic waste.*

OAT is frequently used in integrative pediatrics to help understand digestive imbalance, nutritional needs, and detoxification support in children.*

Limitations: Some markers may fluctuate or may not reflect certain patterns if compounds are only intermittently excreted.

Selected OAT Findings

Neurotransmitter-Related Metabolites

Marker	Baseline	3 Months	Reference Range	% Improvement
HVA	14	3.5	0.49–13	75%
VMA	4.5	1.5	0.72–6.4	69%
HVA/VMA Ratio	3.2	2.5	0.23–2.8	22%
5-Hydroxyindoleacetic Acid	108	0.54	<11	99.5%

Detoxification Support Indicators

Marker	Baseline	3 Months	Reference Range	% Improvement
Pyroglutamic Acid	79	25	13–62	68%
Orotic Acid	1.3	0.87	0.04–0.80	33%
2-Hydroxyhippuric Acid	1.8	0.97	<1.2	46%

Understanding These Shifts

• Neurotransmitter-related metabolites (HVA/VMA): Improvements in these markers reflect healthier stress responses and more balanced neurochemical activity.* Parents often notice this as calmer behavior or clearer communication.*

• Pyroglutamic Acid (Glutathione marker): A reduction indicates better support for glutathione recycling and natural detoxification pathways.*

• Orotic Acid & 2-Hydroxyhippuric Acid: Shifts in these markers show improved handling of everyday metabolic byproducts and environmental exposures.*

These types of changes translate into more consistent energy, easier transitions, and improved behavior throughout the day.*

Intake Details

The child continued his usual diet, including high-oxalate foods such as nuts. CytoFlora® was started immediately after the baseline OAT. Intake gradually increased over two weeks to 5 droppers, twice daily, mixed in water or juice. This was maintained for approximately 2½ months.

CytoFlora® is a liquid probiotic lysate formula that provides beneficial probiotic fractions without introducing live bacteria. This approach is often well-suited for children and sensitive individuals who need gentle digestive and immune support.*

References

Great Plains Laboratory / Mosaic Diagnostics: Organic Acids Test – applications in pediatric gut balance, mitochondrial function, and detoxification.
Great Plains Laboratory: HPHPA and Clostridia-related metabolites.
Kazek B, et al. Intestinal microflora of autistic children. Med Dosw Mikrobiol. 2010;62(3):237–243.
Fungi and allergic lower respiratory tract diseases. J Allergy Clin Immunol. 2012;129(2):280–291.
OAT interpretation guides covering yeast/fungal markers, oxalates, mitochondrial metabolites, and neurotransmitter-related compounds.